Fig. 1

miR-8084 is up-regulated in breast cancer and promotes the clonogenicity of breast cancer cells. a miR-8084 in serum of breast cancer and that of non-cancer samples was analyzed using data from GEO (GSE73002) (**P < 0.01). b miR-8084 was detected in breast cancer tissues. The data is shown as ^−△△CT values. c Relative expression of miR-8084 in breast cancer tissues compared with normal tissues. Data is shown as Mean ± SEM (**P < 0.01). d The effect of miR-8084 on clonogenicity was evaluated by the plate colony formation assay. BT-549 cells were transfected with miR-8084 mimics or control, seeded onto six-well plates. The number of colonies was counted on the 14th day after seeding. e MDA-MB-231 cells were transfected with miR-8084 inhibitor or control then seeded onto six-well plates. The number of colonies was counted on the 14th day after seeding. f, g Colonies containing 50 or more cells were counted. Results are means of three independent experiments ± SD (*P < 0.05)