Fig. 1

Representative flow-FISH analysis of TL in monocyte subsets from an ambulatory patient with HF. Cells were previously selected by G0/G1 DNA Content, scatter properties and CD86 and CD15 markers for monocytes and 1301 cell line selection as described in Materials and methods (data not shown). Monocyte subsets were analyzed based on their CD14 and CD16 expression (a: 1. CD14⁺⁺CD16⁻; 2. CD14⁺⁺CD16⁺; 3. CD14⁺CD16⁺). Scatter properties of 1301 cell line and gating was shown in b. Hybridization of FITC-labeled Telomere Probe (PNA; color filled histograms) and control (without probe; empty histograms) in the three existing monocyte subsets and internal reference control 1301 cell line c. The relative TL value for each monocyte subset was finally calculated as the ratio between the MFI of each subset and the MFI of the control cells. Corrections were also made for the DNA index of G0/G1 cells