Generation of GHR-modified pigs as Laron syndrome models via a dual-sgRNAs/Cas9 system and somatic cell nuclear transfer

Table 1 Development of reconstructed GHRKO cloned embryos after transfer to recipient gilts

Recipients	Donor cell	No. of transferred embryos	Pregnancy (%)	Days of pregnancy	No. of offspring (alive)
1	C3	236	+	121	3 (3)
2	C6	200	+	118	1
3		200	+	118	3 (3)
4		210	−
5		210	−
6^a		350	+	63	2 (1), fetuses
7	Recloning with alive fetus derived from C6	220	−
8		215	+	119	1
9		215	−
10		210	+
11		220	+	118	2 (1)
12		210	+
13		157	+	121	4 (2)
14		210	−
15^a		210	+	117	1 (1)
Total		218.2 ± 40.1	10 (66.7%)		15 (10)

^aThe pregnant recipients #6 and #15 determined by b-scan returned to estrous 63 days and 49 days after embryo transfer, respectively. The living fetus from recipient #6 was used for the isolation of fibroblast for recloning. The fetus detected by surgery in recipient #15 developed until birth 117 days after embryo transfer

ISSN: 1479-5876