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Fig. 4 | Journal of Translational Medicine

Fig. 4

From: Generation of GHR-modified pigs as Laron syndrome models via a dual-sgRNAs/Cas9 system and somatic cell nuclear transfer

Fig. 4

Evaluation of GHR expression in GHRKO pigs. a Immunofluorescence analysis of GHR in fibroblasts isolated from GHRKO piglets. Fibroblasts were stained with anti-GHR secondary antibodies (red). DAPI (blue) staining indicates the nucleus. The data are representative of at least three independent experiments. b Relative expression of GHR mRNA in tissues. Relative GHR expression was detected in heart, liver, kidney and muscle tissues. GAPDH served as the internal control. The data were derived from four GHRKO pigs and three wildtype pigs; the bars represent the mean ± SEM; *P < 0.05 and **P < 0.01. c Immunoblotting analysis of porcine GHR in heart and kidney tissues. Protein extracted from wild-type pig was used as the control. β-Actin was used as the internal control. d Immunochemical analysis of various tissues from GHRKO pigs. Heart, liver, kidney, spleen, testis and muscle tissues from GHRKO pigs were GHR-deficient. Wild-type pigs were used as the control. e Compared to those in control pigs, serum IGF-I and blood glucose levels were significantly decreased in GHRKO pigs, which was consistent with that observed in Laron patients; **P < 0.01

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