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Fig. 2 | Journal of Translational Medicine

Fig. 2

From: Generation of GHR-modified pigs as Laron syndrome models via a dual-sgRNAs/Cas9 system and somatic cell nuclear transfer

Fig. 2

Highly efficient DNA deletion mediated by dual-sgRNAs/Cas9. a Detected modified GHR sequences of 40 alleles in 20 single-cell colonies. The five types of mutations detected include − 47, − 46, − 49, − 18 and − 1 bp. WT: wild-type sequence. “–”: deletion. N/40: Modified GHR alleles detected out of 40 total alleles. b Percentage of GHR mutation types detected in 40 alleles. c Percentage of DNA fragment deletion. d Detection of sgRNA1 and sgRNA2 programmed Cas9 targeting effect by T7ENI cleavage assay. e The mutant genotype of GHR mediated by sgRNA1/Cas9 or sgRNA2/Cas9 system. The red font indicates the sgRNA1 targeting site, and the green font indicates the sgRNA2 targeting site

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