Fig. 2

DN B cells are inversely correlated with the presence of affinity-matured B cells. a Tumor samples were first gated for single cells and then for APC-CD79A expression. The percentage of each subset within this population is defined by PE-IgD and FITC-CD27 expression as follows: CD27⁻IgD⁻ (DN) B cells, CD27⁺IgD⁻ Affinity-matured B cells, CD27⁻IgD⁺ Naïve (non-switched) B cells, and CD27⁺IgD⁺ Early memory/GC (germinal center) B cells. The data are presented according to ascending amounts of DN B cells. b The percent of CD27⁻IgD⁻ DN B cells (x-axis) and percent of CD27⁺IgD⁻ Affinity-matured B cells (y-axis) were defined as described above and plotted for each patient in ranked order. Spearman’s rank correlation coefficient was determined to be − 0.76 with a p-value of 0.001