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Table 1 Comparison of primary BMSCs cultured with 10% HPGF-C18 and 20% FBS from marrow aspirates of 5 healthy subjects

From: Comparison of human bone marrow stromal cells cultured in human platelet growth factors and fetal bovine serum

  Media supplement Number of nucleated cell from marrow Total number of BMSCs harvested Days in culture CFE per 1 × 105 cells
BM01 10% HPGF-C18 10.0 × 106 1.20 × 105 14 2
20% FBS 10.0 × 106 1.14 × 106 14 1
BM03 10% HPGF-C18 6.10 × 106 3.00 × 105 12 4a
20% FBS 6.10 × 106 4.20 × 105 12 3
BM04 10% HPGF-C18 55.2 × 106 7.8 × 105 13 5a
20% FBS 55.2 × 106 5.94 × 106 13 4
BM06b 10% HPGF-C18 5.8 × 106 2.2 × 106 12 9a
20% FBS 5.8 × 106 3.78 × 106 12 11
BM07b 10% HPGF-C18 21.7 × 106 2.4 × 106 12 30a
20% FBS 21.7 × 106 1.14 × 106 12 25
  1. aColonies with atypical features
  2. bParticles were allowed to settle for the preparation of HPGF-C18
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Journal of Translational Medicine

ISSN: 1479-5876

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