Fig. 7

HCMV-FcRs can be specifically recognized by mutated CH2–CH3 domains. Secretion of IFN-γ from different CAR T cells co-cultured for 4 h with a HCMV-infected HFF (AD169; MOI 5; 4 days p.i.) and b non-infected HFF. CH2–CH3 dependence of CAR T cell activation was analyzed by pre-incubation of the HFF with 10% human serum (final concentration) as indicated. IFN-γ levels were normalized for each donor to the value obtained in absence of human serum in the co-culture of T cells expressing the CAR with the wild-type CH2–CH3 domain (1.0–3.2 ng/ml) (n = 3, three different donors; ratio paired two-tailed Student’s t test)