Fig. 5

HCMV-FcR expressed in infected HFF can trigger CAR T cells. IFN-γ secretion from gB- and CEA-specific CAR T cells after co-culture with non-infected HFF or HFF 4 days after infection (AD169; MOI 5) or a IIA1.64 cells. CH2–CH3 dependence of CAR T cell activation was analyzed by the addition of 10% human serum as indicated. IFN-γ levels were normalized for each donor to the value obtained with the co-culture of gB-specific CAR T cells with IIA1.64 cells (mean ± standard deviation; n = 3, three different donors; ratio paired two-tailed Student’s t test)