Fig. 3
Blockade of IFN-γ and TNF neutralizes the inhibitory effect of CAR T cells. The supernatants of the gB-specific CAR T cells co-cultured with infected HFF (which were already used for the experiments shown in Figs. 1c and 2) were added to fresh HFF concomitantly with HCMV (AD169; MOI 0.3) and different blocking antibodies or isotype antibodies as indicated (30 µg/ml each). The same antibodies were added again on day 2 after infection. The percentages of infected HFF (GFPpos) were determined by flow cytometric analysis on day 4 (n = 4; two-tailed Student’s t test)