Fig. 3

Complexity score of peripheral blood TCRVα and TCR-Vβ repertoire in our patient at the 1st, 2nd and 3rd immune monitoring as well as in healthy controls (HC). The complexity of Vβ- and Vα-chain repertoires was determined by counting the number of peaks in spectratype analysis. A score of 8 describes a normal CDR3 size variability of 8–10 peaks per Gaussian curve, a score of 1 refers to profiles showing single peak, 0 describes the absence of peaks. The overall TCR complexity (complexity score) is the sum of 26 individual TCR Vβ- or 34 TCRVα family scores respectively (with a maximum of 26 × 8 = 208 for the β-, and 34 × 8 = 272 for the Vα families)