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Fig. 6 | Journal of Translational Medicine

Fig. 6

From: High expression of TMEM40 is associated with the malignant behavior and tumorigenesis in bladder cancer

Fig. 6

TMEM40 shRNA suppresses the expression of TMEM40 in bladder cancer cells in vitro and tumorigenicity in vivo. a The knockdown vector hU6-MCS-CMV-GFP-SV40-Neomycin was engineered to express shRNA under the U6 promoter. c The expression of green fluorescent protein after 48 h of shRNA vector/NC vector transfection in 5637 and EJ cells (magnification ×100). The shRNA significantly downregulated the expression level of TMEM40 in 5637 and EJ cells. b Protein. d mRNA. e Representative images of tumorigenicity assay performed in nude mice. f Tumor weight was measured after sacrifice at the end of the experiment. g Tumor volume was monitored by caliper measurements twice weekly. All results were presented as the mean ± SD from 3 independent experiments (*P < 0.05, **P < 0.01, ***P < 0.001)

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