Fig. 6From: High expression of TMEM40 is associated with the malignant behavior and tumorigenesis in bladder cancerTMEM40 shRNA suppresses the expression of TMEM40 in bladder cancer cells in vitro and tumorigenicity in vivo. a The knockdown vector hU6-MCS-CMV-GFP-SV40-Neomycin was engineered to express shRNA under the U6 promoter. c The expression of green fluorescent protein after 48 h of shRNA vector/NC vector transfection in 5637 and EJ cells (magnification ×100). The shRNA significantly downregulated the expression level of TMEM40 in 5637 and EJ cells. b Protein. d mRNA. e Representative images of tumorigenicity assay performed in nude mice. f Tumor weight was measured after sacrifice at the end of the experiment. g Tumor volume was monitored by caliper measurements twice weekly. All results were presented as the mean ± SD from 3 independent experiments (*P < 0.05, **P < 0.01, ***P < 0.001)Back to article page