Fig. 4

Effects of upregulation or downregulation of TMEM40 on cell proliferation in bladder cancer cells. Cell proliferation was determined by CCK-8 and EdU assay. Cell proliferation promotion was observed in bladder cancer 5637 cells (a), EJ cells (c). Cell proliferation inhibition was observed in bladder cancer 5637 cells (b), EJ cells (d). Whereas TMEM40 overexpression promote 5637 (e, f) and EJ cells (g, h) proliferation. Data are shown as mean ± SD (magnification ×100,*P < 0.05, **P < 0.01, ***P < 0.001)