Fig. 3
Overexpression of TMEM40 promotes bladder cancer cell migration and invasion. Cell migration and invasion was determined by wound healing and transwell assay. a, b Wound healing assay showed that TMEM40 shRNA resulted in a slower closing of scratch wounds was observed in bladder cancer 5637 and EJ cells. c, d Transwell invasion assay was measured and the results were expressed as the number of invaded cells per field compared with respective control. Data are expressed as the mean ± SD (magnification ×100, *P < 0.05, **P < 0.01, ***P < 0.001)