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Fig. 3 | Journal of Translational Medicine

Fig. 3

From: Comparison of membrane affinity-based method with size-exclusion chromatography for isolation of exosome-like vesicles from human plasma

Fig. 3

Protein analysis of EVs isolated by exoEasy kit and SEC qEV columns. a Western blotting analysis of proteins considered as exosome markers, non-exosomal proteins and albumin. As controls, cell lysate (CL) of P3HR-1 lymphoblastoid cell line and plasma ELVs prepared by ultracentrifugation (UC; based on protocol described by Thery et al [30]) are shown. Representative donor of six analyzed is shown. b Total protein content. c Particles to protein ratio and d total lipid content of EV preparations from an original set of plasma samples from six healthy donors, as described in “Methods”. (mean ± SEM, n = 6; *p < 0.05, **p < 0.01, Wilcoxon matched-pairs signed rank test). e Total lipid content of EV preparations from a second set of plasma samples from six healthy donors and three lymphoma patients, as described in Methods. (mean ± SEM, n = 3)

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