Fig. 1

TEM analysis of EVs isolated by exoEasy kit and SEC qEV columns. Negative staining using uranyl acetate. a Representative images of EV isolated from the same donor. ExoEasy samples were diluted 1/20. Magnification ×5000 and ×10,000; scale bar 500 nm (qEV and right image of exoEasy) and 200 nm (left image of exoEasy). b Diameter of isolated particles. A total of 50 particles were analyzed in at least three independent images per donor. Particles with diameter of > 20 nm were measured. (mean ± SEM, n = 6; *p < 0.05, Wilcoxon matched-pairs signed rank test)