Fig. 1

The level and clinical significance of MEG3 methylation in retinoblastoma. a The methylated (160 bp) and unmethylated (120 bp) PCR products were designated as M or U, respectively. Retinoblastoma tissues C1–C5 and corresponding non-tumor tissues N1–N5 was used as examples. b The frequency of methylation of MEG3 promoter in retinoblastoma tissues and corresponding non-tumor tissues was shown. c Correlation of promoter methylation and MEG3 expression in retinoblastoma. d and e Kaplan–Meier plots for patient’s survival according to the methylation status of MEG3 promoter. The log-rank test was used to calculate the P value. **P < 0.01