Design and validation of a disease network of inflammatory processes in the NSG-UC mouse model

Table 2 Challenge with ethanol resulted in increased expression gene associated with inflammation

Feature ID	Range	IQR	Difference	Fold change	p value
Cytokine
Il17c	7	2	2.5	11	0.011
Il1rn	3090	1724	718.75	1.29	0.002
Il33	712	483	− 347	− 1.98	0.002
Il34	326	132	81.5	1.44	0.007
Il7	179	120	39.5	1.20	0.027
Tgfb1	243	70	63.25	1.45	0.036
Tnf	100	57	49.25	4.65	2E−07
Tslp	39	22	10.5	1.39	0.062
Growth factor
Fgf11	248	71	− 95.75	− 1.65	0.013
Fgf9	111	83	− 57	− 1.57	0.018
Igf1	1152	431	412	1.91	1E−04
Phospholipase
Pla2g10	2170	1285	341.25	1.16	0.039
Pla2g2f	1314	496	349.75	1.33	0.005
ECM
Col1a1	11,375	2581	4058	2.26	1E−08
Metalloproteinase
Mmp14	2092	312	533.5	1.53	0.010
Mmp2	3247	1104	611.5	1.30	0.045
Cell survival/apoptosis
Casp7	2691	1749	468	1.16	0.028
Card9	35	10	9	1.64	0.044
S100a14	4618	1785	1125.5	1.29	0.002
S100a16	5359	1594	924	1.21	0.033
Metabolism
Pparg	1021	374	354	1.54	7E−05

Sections from distal parts of the colon of NSD mice reconstituted with PBMC derived from a patient with UC were challenged with ethanol and subjected to RNAseq analysis. Unchallenged control (control, n = 4), group challenged with 10% ethanol at day 8, and 50% ethanol at days 15 and 18 (ethanol, n = 4). Expression values are depicted as mean total exon read count/mapped reads [million] * exon length [kb] (RPKM). The difference between the highest and the lowest expression value over all samples (range), inter-quantile rage of expression values (IQR), difference between the mean of expression values of the ethanol challenged group and the control group, mean expression value of ethanol challenged group divided by the mean expression value of the control group, FDR corrected p value (EDGE test) (p value)

ISSN: 1479-5876