Table 1 The output and input metabolite for the shortlisted 33 each genes

Targets involved in nucleic acid transactions

 Purines metabolism

  dfrA

7,8-dihydropteroate

Tetrahydrofolate

  folB

7,8-dihydroneopterin

6-hydroxymethyl-7,8-dihydropterin

 Pyrimidines metabolism

  pyrF

Phosphoribosyl pyrophosphate

Phosphoribosyl amine

  Tmk

2′-Deoxyuridine 5′ diphosphate/2′-deoxyuridine 5′-phosphate/deoxythymidine 5′-diphosphate/thymidine monophosphate

2′-Deoxyuridine 5′-diphosphate/2′ deoxyuridine 5′-phosphate/deoxythymidine 5′-diphosphate/thymidine monophosphate

 Nucleotide metabolism

  rpiB

Ribose-5-phosphate/ribulose-5-phosphate

Ribose-5-phosphate/ribulose-5-phosphate

  Dcd

dCTP/dUTP

dCTP/dUTP

  atpE

ADP

ATP

  nrdI

met-NrdF_ox

met-NrdF_red

 DNA replication

  nrdF2

Ribonucleotides

Deoxyribonucleotides

 RNA pseudouridine synthesis

  Rv1711

Pseudouridineguide snoRNAs (Pseudouridine)

RNA pseudouridine

Targets involved in membrane biosynthesis

 Fatty acid metabolism

  fcoT

Acyl-ACP

Fatty acids

  acpM

FASII complex

AcpM (FAS-II complex)

  desA2

Stearoyl-CoA (saturated fatty acids)

oleoyl-CoA (unsaturated fatty acids)

  echA3

Δ2-enoyl-CoA

3-hydroxyacyl-CoA

  echA18.1

Δ2-enoyl-CoA

3-hydroxyacyl-CoA

Targets involved in carbohydrate metabolism

 Kerb cycle

  Carbohydrate metabolism

   pntAb

Ethanol/citrate/Fd ²⁻_red

Acetyl-CoA/2-oxoglutarate/Fd_ox

   nuoA

NADH

NAD⁺

   canB

CO₂

Bicarbonate

  Electron transport cycle

   ctaE

Cytochrome_red

Cytochrome_ox

   Rv0763c

NADP⁺ reductase_ox

Ferredoin NADP⁺ reductase_red

   nrdH

CDP/UDP

dCDP/dUDP

  Mycothiol biosynthesis

   Mca

(Mycothiol (MSH)/MS-electrophiles (MSR)

AcCys + GlcN-Ins AcCySR (N-acetyl-CyS-conjugate)/(mercapturic acid) + GlcN-Ins

Targets involved in de novo pathways

 Essential cofactors

  kdtB

4′-phosphopantetheine

3′-desphospho-coenzyme A

  Rv2361c

Isopentenyldiphosphate

Decaprenyldiphosphate

  Mog

Molybdopterin

Adenylatedmolybdopterin

  moaD2

Cyclicpyranopterin monophosphate/molybdopterin converting factor

Molybdopterin/molybdenum cofactor

 Vitamin biosynthesis

  pdxH

Pyridoxamine 5′-phosphate

Pyridoxal 5′-phosphate

 Amino acid biosynthesis

  prsA

Ribose-5-phosphate

5-phospho-α-d-ribose 1-diphosphate

  Gap

D-glyceraldehyde 3-phosphate

3-phospho-d-glyceroyl phosphate

 Peptide metabolism

  dapE

CysGly + Glu/N-succinyl-ll-2, 6-diaminoheptanedioate

Cys + Gly/succinate + ll-2,6-diaminoheptanedioate

 Carbon, nitrogen and sulfur metabolism

  Rv3600c

Pantothenate

4′-phosphopantothenate