Fig. 1

PRDX2 is a target of miR-200b-3p. a Wenn diagram of prediction for possible miRNAs targeting PRDX2 by three bioinformatic databases. b PRDX2 protein levels in six CRC cell lines were detected by western blot and quantified by Image J software. The grey value of PRDX2 was normalized to that of the corresponding GAPDH. c Expression levels of miR-200b-3p, miR-200c-3p, miR-1228-3p and miR-429 were detected in six CRC cell lines by qPCR. The relative expression levels were normalized to Caco2 cell (*p < 0.05 compared to LoVo cell,^# p < 0.05 compared to SW620 cell). d Pearson correlation analysis of PRDX2 protein levels with miR-200b-3p, miR-200c-3p, miR-1228-3p and miR-429 expression in six CRC cell lines. e Predictive binding sites and mutant sites of miR-200b-3p to 3′UTR of PRDX2 mRNA. f The luciferase activities of wild-type and mutant-type pmirGLO-3′UTRs of PRDX2 in 293T and LoVo cells after transfection of miR-200b-3p mimics (***p < 0.001, RL Ranilla luciferase, FL Firefly luciferase). g PRDX2 protein levels were detected by western blot in SW620 and LoVo cells after transfection of miR-200b-3p mimics. The grey value of PRDX2 was normalized to that of the corresponding GAPDH