Table 3 NGS to detect novel mutations or less frequently mutant genes
From: Next-generation sequencing: recent applications to the analysis of colorectal cancer
Samples | NGS | # analyzed genes | Novel mutations/less frequently mutant genes | Ref. |
|---|---|---|---|---|
60 pairs normal/CRC | GAIIx Illumina | 183 Targeted-seq (custom panel) | 166-point mutations 25 indels | Han et al. [90] |
4 mCRC | Illumina HiSeq | Whole genome (NEBNext DNA New England) | INPPL1 p.E567G | Shanmugam et al. [91] |
77 CRC | Ion Torrent | 22 Targeted-seq (ampliseq colon and lung) | ALK p.L1196M/AKT1, STK11, ERBB2, ERBB4, MAP2K1, NOTCH1 | Malapelle et al. [92] |
14 HNPCC, 12 EC, 2 LS | Illumina HiSeq | 22 Targeted-seq (MMR custom panel) | POLD2, EXO1 | Talseth-Palmer et al. [93] |
224 CRC | Illumina HiSeq | 341/410 cancer associated genes (Msk-impact) | POLE p.P286R | Stadler et al. [94] |
9643 mCRC | Ion Torrent | 50 Targeted-seq (Ampliseq cancer hotspot) | Non-V600 BRAF | Jones et al. [95] |