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Table 1 Primer sequences for PCR amplification of each gene studied

From: Phenolic compounds apigenin, hesperidin and kaempferol reduce in vitro lipid accumulation in human adipocytes

  Sense primer Antisense primer
c/ebpβ 5′-GGCAGCACCACGACTTCCT-3′ 5′-CGCCCCAGGCTCACGTAG-3′
c/ebpα 5′-AGGGTCTCTAGTTCCACGCC-3′ 5′-CAAGGGGAAGCCCAGCCTATA-3′
pparγ 5′-TAGATGACAGCGACTTGGCAATAT-3′ 5′-GAATGTCTTCAATGGGCTTCACA-3′
srebp1c 5′-ACGCCCCACTTCATCAAGG-3′ 5′-ACTGTTGCCAAGATGGTTCCG-3′
acc 5′-CATCAGCAGAGACTACGTCCTCAA-3′ 5′-CATGGCAACCTCTGGATTGG-3′
perilipin 5′-TGGAGACTGAGGAGAACAAG-3′ 5′-ATGTCACAGCCGAGATGG-3′
scd1 5′-GCAGGACGATATCTCTAGCT-3′ 5′-GTCTCCAACTTATCTCCTCCATTC-3′
atgl 5′-GTGTCAGACGGCGAGAATG -3′ 5′-TGGA GGGAGGGAGGGATG-3′
hsl 5′-TCAGTGTCTAGGTCAGACTGG-3′ 5′-AGGCTTCTGTTGGGTATTGGA-3′
fasn 5′-TATGCTTCTTCGTGCAGCAGTT-3′ 5′-GCTGCCACACGCTCCTCTAG-3′
dgat 5′-ATTGCTGGCTCATCGCTGT-3′ 5′-GGGAAAGTAGTCTCGAAAGTAGC-3′
18S 5′-TTCGAACGTCTGCCCTATCAA-3′ 5′-ATGGTAGGCACGGCGACTA-3′
  1. c/ebpβ, CCAAT/enhancer-binding protein beta; c/ebpα, CCAAT/enhancer-binding protein alpha; pparγ, peroxisome proliferator fator gamma; srebp1c, sterol regulatory element-binding protein 1c; acc, acetyl-CoA carboxylase; scd1, stearoyl-CoA desaturase 1; atgl, adipose triglyceride lípase; hsl, hormone sensitive lípase; fasn, fatty acid synthase; dgat, diglyceride acyltransferase