Fig. 3
Loss of PTHLH significantly suppressed cell proliferation. a–c PTHLH deletion suppresses tumor growth. Five nude mice were injected subcutaneously with 1 × 107 cells/mouse for each of the indicated stable cell lines. The results are presented as isolated tumors (a), tumor sizes (b), and tumor weights (c). d H&E staining of xenograft tumor from nude mice injected with shCtrl or shPTHLHx cells (scale bar, 20 μm). e Representative images of Ki67, CyclinD1, and CDK4 protein expression in xenograft tumors from nude mice as assessed by immunohistochemistry (DAB staining, scale bar, 50 μm)