GMP-production of purified human B lymphocytes for the adoptive transfer in patients after allogeneic hematopoietic stem cell transplantation

Table 2 Summary of GMP separations

Number	Fractions	CD45+ cells (× 10⁸)	CD19+ cells (%)	CD19+ cells (× 10⁸)	CD3+ cells (%)	CD3+ cells (× 10⁸)	Recovery of B cells (%)^a
1	Leukapheresis before thrombowash	184.5 ± 73.08^b	13.62 ± 5.27	24.40 ± 14.80	49.01 ± 6.41	87.76 ± 38.36	100
2	After thrombowash	161.84 ± 66.92	13.2 ± 4.93	21.19 ± 13.25	49.02 ± 12.85	78.28 ± 35.79	89.6 ± 16.8
3	After binding of CD3 beads	114.18 ± 65.94	17.2 ± 6.19	17.37 ± 9.33	32.35 ± 15.78	37.18 ± 37.27	80.5 ± 37.3
4	Target-fraction after CD3 depletion	58.25 ± 37.62	24.63 ± 10.47	13.18 ± 10.0	0.16 ± 0.27	0.23 ± 0.70	50.4 ± 17.3
5	Non-target fraction after CD3 depletion	43.81 ± 29.64	10.06 ± 5.99	3.15 ± 1.94	57.14 ± 22.08	27.45 ± 25.39	16.4 ± 10.6
6	After binding of CD19 beads	48.65 ± 32.57	17.77 ± 6.98	6.26 ± 4.58	0.14 ± 0.28	0.07 ± 0.13	27.1 ± 13.4
7	Target-fraction after CD19 enrichment	5.04 ± 3.77	95.98 ± 3.31	4.70 ± 3.64	0.10 ± 0.20	0.005 ± 0.006	18.9 ± 10.6
8	Non-target fraction after CD19 enrichment	33.42 ± 14.49	0.34 ± 0.45	0.10 ± 0.15	0.16 ± 0.33	0.07 ± 0.13	0.39 ± 0.68

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