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Fig. 2 | Journal of Translational Medicine

Fig. 2

From: GMP-production of purified human B lymphocytes for the adoptive transfer in patients after allogeneic hematopoietic stem cell transplantation

Fig. 2

Immunophenotype of individual fractions from the two-step separation protocol. Density plots of combined stainings with CD45 PerCP, CD20-FITC and CD3-APC antibodies. 1 leukapheresis before thrombowash; 2 after thrombowash; 3 after binding of CD3 beads; 4 target fraction after CD3 depletion; 5 non-target fraction after CD3 depletion; 6 after binding of CD19 beads; 7 target fraction after CD19 enrichment; 8 non-target fraction after CD19 enrichment. Flow cytometry density plots of one representative separation performed under GMP conditions are shown

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Journal of Translational Medicine

ISSN: 1479-5876

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