Fig. 1

Comparison of one-step and two-step separation strategy. a Purity of CD19⁺ B cells in %; b absolute yield of B cells; c contamination of B-cell product with CD3⁺ T-lymphocytes in %; d absolute numbers of T-lymphocytes in the B-cell product; e recovery of B-lymphocytes in % of the starting leukapheresis material. Box plots figures for one-step (CD19⁺ selection; n = 5) and two-step (CD3⁻ selection followed by CD19⁺ selection; n = 6) separations are shown; *p < 0.05 (Mann–Whitney), ns not significant