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Table 1 Summary of the methods used for patient-derived xenografts of primary tumor cell cultures in zebrafish

From: Management and potentialities of primary cancer cultures in preclinical and translational studies

Tumor origin Origin of species Sample Zebrafish line No. of cells Stage Site of injection References
Abdominal liposarcoma Human SR Tg(Kdrl:mCherry) 50–400 Embryo (2 dpf) Heart cavity [105]
Acinar cell carcinomas Zebrafish SR tg(CB1) 2/3 × 105 and fragments Larvae (7–14 dpf)/adult Intraperitoneal/abdomen and dorsal muscles [159]
Acute lymphoblastic leukemia Zebrafish SR AB and EK 5 × 105 Adult Intraperitoneum [160]
Acute myeloid leukemia Human BM AB 100–200 Blastula (3 hpf) Yolk sac [161]
Ampulla of Vater adenocarcinoma Human SR Tg(fli1:eGFP)/alb(albinos)   Embryo (2 dpf) Yolk sac [162]
Ampulla of Vater adenocarcinoma Human SR Tg(fli1:eGFP) Fragments Embryo (2 dpf) Yolk sac [163]
Bone metastasis Human SR Tg(fli1:eGFP) 50–400 Embryo (2 dpf) Duct of Cuvier [72]
Colon adenocarcinoma Human SR Tg(fli1:eGFP)/alb(albinos)   Embryo (2 dpf) Yolk sac [162]
Ependymoma Mouse SR Tg(fli1:eGFP) 2 × 105 Juvenile (30 dpf) Cerebral hemisphere [164]
Glioblastoma Human SR AB   Embryo (2 dpf)/adult Brain ventricle [165]
Glioblastoma Mouse SR Tg(fli1:eGFP) 2 × 105 Juvenile (30 dpf) Cerebral hemisphere [164]
Liver metastasis from NET Human SR Tg(fli1:eGFP) 100 Embryo (2 dpf) Perivitelline space [166]
Melanoma Zebrafish SR Casper 2 × 105 Adult Peritoneal cavity/intracardiac cavity [167]
Multiple myeloma Human Pl Casper 100 Embryo (2 dpf) Perivitelline space [168]
Myeloid leukemia Human PB AB 50–200 Embryo (2 dpf) Posterior cardinal vein [169]
Pancreatic cancer Human SR Tg(fli1:eGFP)/alb(albinos)   Embryo (2 dpf) Yolk sac [162]
Pancreatic cancer Human SR Tg(fli1:eGFP) Fragments Embryo (2 dpf) Yolk sac [163]
Papillary thyroid cancer Human SR Tg(fli1a:EGFP)y1 100 Embryo (2 dpf) Perivitelline space [170]
Pituitary adenoma Human SR Tg(fli1:eGFP) 100 Embryo (2 dpf) Perivitelline space [166, 171, 172]
Prostate cancer Human SR Casper   Embryo (2 dpf)/juvenile Sinus venous/subcutaneous injection [173]
Rhabdomyosarcoma Zebrafish SR AB 10–2 × 104 Adult Intraperitoneal cavity [174]
Stomach adenocarcinoma Human SR Tg(fli1:eGFP)/alb(albinos)   Embryo (2 dpf) Yolk sac [162]
Testicular germ cell tumor Zebrafish SR AB/TU 5 × 103 Adult Intraperitoneal cavity [175]
  1. SR surgical resection, BM bone marrow, PB peripheral blood, Pl plasma