Fig. 2From: Efficient generation of P53 biallelic knockout Diannan miniature pigs via TALENs and somatic cell nuclear transferTALEN-mediated P53 mutations in PFFs. a Identification of P53-mutant cell lines. Cell lines showing one band indicate the WT allele, while mutated alleles produced three bands in a T7EI assay. b The sequences of P53-mutant cell lines. The WT sequence is shown above. Deletion and point mutations (denoted with “Δ” and “p” with the number of base pairs) are identifiedBack to article page