Fig. 5

Quantification of the CD8⁺T:Foxp3 and CD8⁺T:PD-L1 ratios, heatmap generated in Rstudio, and 4-color mIHC images (× 10). a CD8a⁺:Foxp3⁺ ratio. b CD8⁺T:PD-L1⁺ ratio. c Hierarchical clustering of CD8⁺T, Foxp3 and PD-L1. d Scanned image of Sample 9 (10 × ). e Scanned image of Sample 12 (× 10). f Scanned image of Sample 24 (× 10). g Scanned image of Sample 20 (× 10). All images were acquired on a Nikon C1 confocal microscope. h Hierarchical clustering of CD8⁺T:Foxp3 and CD8⁺T:PD-L1. The top region represents the hierarchical clustering results. The number in the lower region represents the sample number