Fig. 1
From: Understanding immune phenotypes in human gastric disease tissues by multiplexed immunohistochemistry
Comparison between IHC staining and 4-color mIHC staining of paraffin-embedded gastric cancer tissue (× 10). a–c IHC staining of CD8 (1:500), Foxp3 (1:350) and PD-L1 (1:250). d, e 4-color mIHC staining of CD8a (1:500), Foxp3 (1:350) and PD-L1 (1:250). DAPI was used to visualize nuclei (blue color), FITC corresponds to PD-L1 (green color), Cy3 represents Foxp3 (yellow color), and Cy5 indicates CD8 (red color)