Fig. 5
From: A Yin-Yang 1/miR-30a regulatory circuit modulates autophagy in pancreatic cancer cells
Ectopic expression of miR-30a decreases YY1-induced autophagy. a Western blotting was performed to quantify the expression levels of LC3I/II and p62 in BXPC-3 and PANC-1 cells after transfection with a miR-30a mimic, a miR-30a inhibitor, or a negative control for 48Â h. b LC3A/B in BXPC-3 and PANC-1 cells after transfection with a miR-30a mimic, a miR-30a inhibitor, or a negative control for 48Â h were detected by Immunofluorescence. c The expression of LC3I/II and p62 in BXPC-3 and PANC-1 cells transfected with YY1-overexpressing lentiviruses or miR-30a mimics were quantified using Western blotting. d The expression of LC3A/B in BXPC-3 and PANC-1 cells transfected with YY1-overexpressing lentiviruses or miR-30a mimics were quantified using Immunofluorescence. Results are representative of at least three independent experiments