Fig. 4

YY1 binds upstream of the miR-30a genomic loci and regulates its expression. a qRT-PCR analysis for miR-30a expression levels in YY1-overexpressing or YY1 knockdown BXPC-3 cells. Results are representative of three independent experiments and are presented as the mean ± SD (bars). *p < 0.05, **p < 0.01, ***p < 0.001. b qRT-PCR analysis for miR-30a expression levels in YY1-overexpressing or YY1 knockdown PANC-1 cells. c Three putative YY1 binding sites upstream of the miR-30a genomic locus predicted using JASPAR bioinformatics software. d A luciferase activity assay was used to determine the effect of YY1 on the expression of a luciferase reporter driven by a miR-30a 3′UTR fragment with or without YY1-specific binding sites in BXPC-3 and PANC-1 cells. e The binding of YY1 to the miR-30a genomic locus was determined by ChIP