Fig. 5

Immunohistochemical preparations. Images and staining densities of histological preparations immunostained for angiopoietin-2 (a), coagulation factor-III (b), tie1 (c), and neuropilin-2 (d). The images show representative examples, refer to a primary tumor of the C-10 melanoma xenograft model and a primary tumor of the T-22 melanoma xenograft model, and illustrate that C-10 tumors showed higher expression of angiopoietin-2, coagulation factor-III, and tie1 and lower expression of neuropilin-2 than T-22 tumors. Staining densities were for each protein normalized to the mean value of the tumor model that showed the highest staining density. C-10, D-12, and E-13 tumors showed higher staining density for angiopoietin-2, coagulation factor-III, and tie1 and lower staining density for neuropilin-2 than N-15, R-18, and T-22 tumors. Columns and bars: mean values ± standard error (n = 5, where n represents the number of studied tumors). The tumors were grown in adult (8–12 weeks of age) female BALB/c nu/nu mice