Fig. 4

Expression of angiogenesis-related genes in two groups of human melanoma xenografts differing in metastatic pattern. A PCR array with 84 genes was used for expression profiling, and the genes on the array are presented in Additional file 1: Table S1. a Expression level in C-10, D-12, and E-13 tumors vs expression level in N-15, R-18, and T-22 tumors. Points: mean values of single genes determined from three different tumors. Solid lines: twofold difference in expression level. b Mean expression level of ANGPT2, F3, NRP2, and TIE1 in C-10, D-12, and E-13 tumors divided by that in N-15, R-18, and T-22 tumors. The mean expression of ANGPT2, F3, and TIE1 was more than twofold higher in C-10, D-12, and E-13 tumors than in N-15, R-18, and T-22 tumors [statistical analysis revealed that all 27 ratios were higher than 2 (p < 0.01)], whereas the mean expression of NRP2 was more than twofold higher in N-15, R-18, and T-22 tumors than in C-10, D-12, and E-13 tumors [statistical analysis revealed that all 9 ratios were lower than 0.5 (p < 0.005)]. The tumors were grown in adult (8–12 weeks of age) female BALB/c nu/nu mice