Fig. 6

Restoration of TDAG8 gene expression reduces U937 cell metastasis in SCID mice. In vivo luminescence imaging identified larger tumors but was not sensitive enough to identify micro metastasis in SCID mice. a IVIS luminescence imaging. Scale bar = 2 cm. b Normal mouse spleen section with negative staining demonstrating the specificity of the anti-human nucleoli antibody. c Tumor metastasis in the lymph node was positive for human nucleoli. d, e Hematoxylin and eosin staining and IHC of the human nucleoli antibody was used to identify nuclear localization of the antibody signals of U937/Vector tumor cells in the lung of SCID mice. f Analysis of SCID mice lungs following IHC of human nucleoli reveals a significant reduction in metastasis. Mice with no metastasis detected were given a score of 0. Mice with < 2 average metastatic lesions in each 5 µm section of the lung were given a score of 1, from 2 to 5 average metastatic lesions were given a score of 2, from 6 to 10 average metastatic lesions were given a score of 3, and from 11 or more average metastatic lesions were given a score of 4. In addition, lesions that were larger than 20 cells or large enough to see with the IVIS Lumina XR unit was given a 4. For each experimental group injected with either U937/Vector-Luc or U937/TDAG8-Luc cells the individual scores were added up and averaged to give a metastasis score. The scoring system consisted of analyzing a total of 6 sections of lung for each mouse injected. Every section was separated by at least 20 µm of tissue to give representative sections of various areas of the lung. **P < 0.01