Fig. 4

Restoration of TDAG8 gene expression reduces c-myc oncogene expression through Gα13/Rho signaling. a IHC demonstrates the majority of cells within the U937/Vector tumors away from necrotic or heterotypic areas are positive for c-myc expression as indicated by DAB chromogen (N = 5). b There were less cells within the U937/TDAG8 tumors away from necrotic or heterotypic areas positive for c-myc expression as indicated by DAB chromogen. Arrowheads indicate cells not expressing c-myc. Observed in at least 3, 5 µm sections of 5 different tumors for each experimental group. c Restoration of TDAG8 in U937 cells reduces c-myc expression in response to treatment with media buffered to physiological pH 7.4 and acidic pH 6.4. d Quantification of c-myc Western blot data displaying the significant inhibition of c-myc mediated by acidosis and TDAG8 gene expression. e, f Gα13/Rho signaling is responsible for a TDAG8 mediated inhibition of c-myc expression. *P < 0.05, **P < 0.01, ***P < 0.001