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Fig. 3 | Journal of Translational Medicine

Fig. 3

From: A lipidated peptide of Mycobacterium tuberculosis resuscitates the protective efficacy of BCG vaccine by evoking memory T cell immunity

Fig. 3

BCG-L91 elicits long lasting memory generation. The mice primed and boosted with BCG-L91 and infected with Mtb were sacrificed. The control animals were immunized with either BCG or placebo. A single cell suspension was prepared from lungs and in vitro incubated with L91, Pam2Cys and ST-CF for 72 h. The gated population of CD4 T cells was checked for the expression of memory markers CD62L, CD44 and CD127 by flow cytometry. a The flow cytometry contours and b bar diagram represents percent population of central memory (CD44hiCD62Lhi) and c effector memory (CD44hiCD62Llo) CD4 T cells of the spleen. d CD4 gated T cells were examined for the expression of CD127 by flow cytometer on spleen cells and e the bar diagram depicts the percentage population of CD127 expressing CD4 T cells. Data shown in the inset are Mean ± SEM and representative of 3 independent experiments. BCG-L91: mice primed with BCG and boosted with L91. *p ≤ 0.05, **p ≤ 0.005, ns not significant

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