Fig. 3

Sustained high expression of BAG-1, and its associated proteins in response to cisplatin resistance. Whole cell lysates of UMSCC 14A, 14B and 17A, 17B cells were treated with indicated concentration of cisplatin or absence for 24, for western blot analysis. a Membranes were probed with antibodies for BAG-1, BCL-xL, BCL-2, BID, and phosphor γH2AX. b Whole cell lysates were also probed for pro-survival pathway, PI3K/AKT, Jak/STAT3, and MAKP/ERK. Each membrane was stripped and re-probed with GAPDH for loading control