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Fig. 6 | Journal of Translational Medicine

Fig. 6

From: Glucocorticoid attenuates acute lung injury through induction of type 2 macrophage

Fig. 6

Influence on Tregs differentiation and function by macrophages. a Induced-M2a and M2c were isolated by flow cytometry and co-cultured with CD4+ naïve T cells, as well as M0 and M1, under Tregs induction environment. CD4+CD25+Foxp3+ Tregs and CD4+IL-17a+RORγt+ Th17 were detected. b Semi-quantitative analysis of Th17 and Tregs percentage showed M1 induced much more percentage of Th17. Compared to M0 and M1, both M2a and M2c significantly induced Tregs and inhibited Th17. Intriguingly, M2c induced more Tregs than M2a. c These M2c-induced Tregs were isolated. Compared to the normal Tregs, the expression of IL-10 and TGF-β in supernatant has no difference in M2c-induced Tregs. d Mix lymphocyte reaction was performed to compare the immunosuppression function between the two groups of Tregs. The immune suppressive function between normal Tregs and M2c-induced Tregs showed no significant difference

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