Table 1 LOD and LOQ (fmol/µg of total protein) of the PRISM-SRM assays for WT and mutant SPOP proteins
Peptide | Charge | Transition | Production type | LOD fmol/µg | LOQ fmol/µg | tR min |
|---|---|---|---|---|---|---|
VNPKGLDEESKDYLSLYLLLVSCPKSEVR | 4+ | 838.7−962.2 | y8 | 0.4 | 1 | 30.0 |
VNPKGLDEESKDYLSLNLLLVSCPKSEVR | 4+ | 826.4−962.5 | y8 | 0.2 | 0.4 | 28.4 |
AKFKFSILNAKGEETKAMESQR | 4+ | 629.1−721.3 | y6 | 0.5 | 1 | 24.5 |
AKCKFSILNAKGEETKAMESQR | 4+ | 632.3−721.3 | y6 | 0.5 | 1 | 23.8 |
FVQGKDWGFKKFIR | 4+ | 439.8−895.6 | y7 | 0.2 | 0.5 | 25.6 |
FVQGKDWGVKKFIR | 4+ | 427.8−847.6 | y7 | 0.1 | 0.2 | 24.5 |
LADELGGLWENSR | 2+ | 730.4−918.4 | y8 | 0.05 | 0.1 | 27.9 |
SLASAQCPFLGPPR | 2+ | 750.9−943.5 | y8 | 0.05 | 0.1 | 26.1 |