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Table 1 LOD and LOQ (fmol/µg of total protein) of the PRISM-SRM assays for WT and mutant SPOP proteins

From: Quantification of mutant SPOP proteins in prostate cancer using mass spectrometry-based targeted proteomics

Peptide Charge Transition Production type LOD fmol/µg LOQ fmol/µg tR min
VNPKGLDEESKDYLSLYLLLVSCPKSEVR 4+ 838.7−962.2 y8 0.4 1 30.0
VNPKGLDEESKDYLSLNLLLVSCPKSEVR 4+ 826.4−962.5 y8 0.2 0.4 28.4
AKFKFSILNAKGEETKAMESQR 4+ 629.1−721.3 y6 0.5 1 24.5
AKCKFSILNAKGEETKAMESQR 4+ 632.3−721.3 y6 0.5 1 23.8
FVQGKDWGFKKFIR 4+ 439.8−895.6 y7 0.2 0.5 25.6
FVQGKDWGVKKFIR 4+ 427.8−847.6 y7 0.1 0.2 24.5
LADELGGLWENSR 2+ 730.4−918.4 y8 0.05 0.1 27.9
SLASAQCPFLGPPR 2+ 750.9−943.5 y8 0.05 0.1 26.1
  1. t R retention time