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Fig. 7 | Journal of Translational Medicine

Fig. 7

From: Tough decoy targeting of predominant let-7 miRNA species in adult human hematopoietic cells

Fig. 7

Let-7 miRNAs focused inhibition modulates the expression levels of BCL11A and HMGA2. Let-7a-TuD effects in a BCL11A, b HMGA2, c ZBTB7A, d KLF1 and e SOX6 mRNA levels. RT-qPCR analyses were performed at culture day 14. Open bars represent control and black bars represent let-7a-TuD. Mean value ± SD of at least four independent donors for each condition. p values were calculated using two-tailed Student’s t-test. f Western blot analyses of BCL11A, HMGA2, ZBTB7A, KLF1 and SOX6 in the nuclear extracts of three independent donors at culture day 14 upon let-7a-TuD compared to control transductions. Blots were probed with anti-BCL11A, anti-HMGA2, anti-ZBTB7A, anti-KLF1 or anti-SOX6 antibodies as indicated. Lamin B1 and Histone H3 were used as loading controls. C, control (negative control vector) transduction; 7a-TuD or 7a, let-7a tough decoy design. *p < 0.05

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