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Fig. 5 | Journal of Translational Medicine

Fig. 5

From: Tough decoy targeting of predominant let-7 miRNA species in adult human hematopoietic cells

Fig. 5

Focused inhibition of let-7 miRNAs reduces in vitro erythroblast enucleation and results in pancellular distribution of HbF. Representative flow dot plots of a let-7a-TuD and control transduction at culture days 14 and 21 stained with anti-transferrin receptor (CD71) and anti-glycophorin A (GPA) antibodies. Culture day 21 cells were also assessed for b enucleation by thiazole orange (TO) staining and c fetal hemoglobin distribution by fetal hemoglobin (HbF) staining for let-7a-TuD and control transduction. Mean value ± SD of five independent donors for CD71, GPA and thiazole orange stains analyses, and of three independent donors for HbF stain. Control, control transduction; let-7a-TuD, let-7a tough decoy design

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