Fig. 3

Let-7 miRNAs focused inhibition by TuD is accompanied by the reduction of mature let-7 levels. Quantitation of copy number per nanogram cDNA (copies/ng cDNA) by RT-qPCR in a let-7a-TuD (n = 5) and b let-7b-TuD (n = 4), both compared to control transductions. c Total levels of mature let-7 miRNAs in let-7a-TuD and let-7b-TuD treatments compared to control. Open bars represent control and black bars represent let-7a-TuD or let-7b-TuD. Mean value ± SD of independent donors for each condition. p values were calculated for comparison of control versus let-7a, let-7b or the total levels of let-7 miRNAs using two-tailed Student’s t-test. TuD, tough decoy design; C, control (negative control vector) transduction; 7a-TuD, let-7a tough decoy design; 7b-TuD, let-7b tough decoy design. *p < 0.05