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Fig. 1 | Journal of Translational Medicine

Fig. 1

From: Coiled-coil domain containing 109B is a HIF1α-regulated gene critical for progression of human gliomas

Fig. 1

CCDC109B is highly expressed in high grade gliomas. a Images of immunofluorescence performed with an antibody against CCDC109B (red) in NHA, U87MG, U251, T98 cells and visualized using confocal microscopy. Nuclei were labeled with DAPI (blue). Scale bar 20 μm. b Western blot analysis of CCDC109B levels in U87MG, U251, T98 and NHA. c mRNA expression levels of CCDC109B as determined using TCGA, CGGA, GSE4271 and Rembrandt databases. d Representative images of IHC staining with anti-CCDC109B antibody on human glioma and non-neoplastic brain tissue samples. Magnification ×200, upper; ×400, lower. e Graphical representation of scoring performed on IHC staining of glioma and non-neoplastic tissue samples for CCDC109B levels. Bar graphs show the mean ± the standard error of the mean (SEM) for each group. f Western blot analysis of CCDC109B in lysates (20 µg) prepared from different grades of human gliomas (WHO grades II–IV) and normal brain tissues (ns not significant, *P < 0.05, ***P < 0.001)

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