Regulation of glucose uptake in lymphoma cell lines by c-MYC- and PI3K-dependent signaling pathways and impact of glycolytic pathways on cell viability

Table 4 Interaction of 2-DG (1 mM) with c-MYC-inhibitor (2 µM; a), LY294002 (5 µM; b), Rapamycin (200 nM; c), PD98059 (5 µM; d) and SB203580 (5 µM; e) in the three lymphoma cell lines

a cell line	2-DG 1 mM	c-MYC-inh. 2 µM	2-DG + c-MYC-inh.	CI
BJAB	89.6 ± 7.4	91.0 ± 7.7	57.7 ± 4.8	1.41 syn.
OCI-LY3	81.0 ± 6.6	85.4 ± 7.3	56.3 ± 5.5	1.23 syn.
SU-DHL-6	84.0 ± 6.3	86.3 ± 6.4	57.9 ± 5.1	1.25 syn.

b cell line	2-DG 1 mM	LY294002 5 µM	2-DG + Y294002	CI
BJAB	88,1 ± 7.0	84.6 ± 7.0	47.2 ± 3.5	1.60 syn.
OCI-LY3	79.0 ± 7.3	73.7 ± 6.6	42.6 ± 4.6	1.37 syn.
SU-DHL-6	83.5 ± 6.4	87.9 ± 8.1	38.2 ± 3.1	1.92 syn.

c cell line	2-DG 1 mM	Rapamycin 200 nM	2-DG + Rapamycin	CI
BJAB	90.7 ± 6.8	90.4 ± 8.3	58.2 ± 3.7	1.41 syn.
OCI-LY3	80.5 ± 6.4	88.9 ± 7.6	69.5 ± 5.0	1.03 add.
SU-DHL-6	82.5 ± 7.6	91.6 ± 7.9	47.8 ± 3.4	1.58 syn

d cell line	2-DG 1 mM	PD98059 5 µM	2-DG + PD98059	CI
BJAB	90.3 ± 8.4	103.7 ± 9.2	95.7 ± 7.7	0.98 add.
OCI-LY3	81.5 ± 6.8	98.2 ± 8.5	75.3 ± 5.6	1.06 syn.
SU-DHL-6	81.5 ± 5.8	99.4 ± 7.8	77.9 ± 6.5	1.04 add.

e cell line	2-DG 1 mM	SB203580 5 µM	2-DG + SB203580	CI
BJAB	91.4 ± 6.7	102.6 ± 9.0	83.5 ± 7.0	1.12 syn.
OCI-LY3	80.8 ± 7.4	97.8 ± 7.6	63,5 ± 5.3	1.24 syn.
SU-DHL-6	79.6 ± 5.7	104.3 ± 8.4	54.1 ± 4.5	1.53 syn.

MTT assays were performed to determine the viability of cells after incubation with one compound alone or in combination. Cl values were calculated according to the method of Drewinko et al. (see “Methods”), where Cl > 1.05 indicates synergism (syn.), 0.95 ≤ Cl ≤ 1.05 indicates additivity (add.) and Cl < 0.95 indicates antagonism

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