Table 1 Components identified from RGE
No. | tR | ID | Molecular formula | [M+HCOO]− or [M−H]− | MS/MS fragmentations in the negative mode with the energy 35 V CID |
|---|---|---|---|---|---|
1 | 35.826 | Noto R3 | C48H82O19 | 1007.5483 [M+HCOO]− 961.5432 [M−H]− | 1007.5483 [M+HCOO]−; 961.5432 [M−H]−; 799.4912 [M−H–Glc]−; 637.4346 [M−H–GlcGlc]−; 475.3805 [M−H–GlcGlcGlc]−; 323.0892 [GlcGlc–H]− |
2 | 37.206 | Noto R1 | C47H80O18 | 977.5363 [M+HCOO]− 931.5303 [M−H]− | 977.5363 [M+HCOO]−; 931.5303 [M−H]−; 799.4886 [M−H–Xyl]−; 637.4339 [M−H–Xyl–Glc]−; 475.3798 [M−H–Xyl–GlcGlc]−; 293.0900 [XylGlc–H2O–H]−; 179.0552 [Glc–H]− |
3 | 39.586 | Rg1 | C42H72O14 | 845.5033 [M+HCOO]− 799.4892 [M−H]− | 845.5033 [M+HCOO]−; 799.4892 [M−H]−; 637.4386 [M−H–Glc]−; 475.3836 [M−H–GlcGlc]−; B1α/B1β 161.0445 |
4 | 40.000 | Re | C48H82O18 | 991.5560 [M+HCOO]− 945.5464 [M−H]− | 991.5560 [M+HCOO]−; 945.5464 [M−H]−; 783.4931 [M−H–Glc]−; 637.4366 [M−H–Glc–Rha]−; 475.3818 [M−H–GlcGlc–Rha]− |
5 | 54.282 | Rf | C42H72O14 | 845.5028 [M+HCOO]− 799.4923 [M−H]− | 845.5028 [M+HCOO]−; 799.4923 [M−H]−; 637.4379 [M−H–Glc]−; 475.3831 [M−H–GlcGlc]−; 391.2842 [M−H–GlcGlc–C6H12]−; 1,3A2β 221.0648; 2,5A1β 101.0244 |
6 | 57.123 | Noto R2 | C41H70O13 | 815.4884 [M+HCOO]− 769.4815 [M−H]− | 815.4884 [M+HCOO]−; 769.4815 [M−H]−; 637.4377 [M−H–Xyl]−; 475.3834 [M−H–Xyl–Glc]−; 391.2905 [M−H–Xyl–Glc–C6H12]− |
7 | 59.815 | Rg2 | C42H72O13 | 829.5027 [M+HCOO]− 783.4934 [M−H]− | 829.5027 [M+HCOO]−; 783.4934 [M−H]−; 637.3811 [M−H–Rha]−; 475.3811 [M−H–Rha–Glc]−; 391.2858 [M−H–Rha–Glc–C6H12]− |
8 | 60.838 | Rb1 | C54H92O23 | 1107.5955 [M−H]− | 1107.5955 [M−H]−; 945.5485 [M−H–Glc]−; 783.4839 [M−H–GlcGlc]− |
9 | 62.804 | Rc | C53H90O22 | 1077.5876 [M−H]− | 1077.5876 [M−H]−; 945.5405 [M−H–Ara(f)]−; 783.4902 [M−H–Ara–Glc]−; 621.4365 [M−H–Ara–Glc–Glc]−; 459.4026 [M−H–Ara–GlcGlcGlc]− |
10 | 63.459 | Ro | C48H76O19 | 955.4959 [M−H]− | 955.4959 [M−H]−; 793.4324 [M−H–Glc]−; 0,2A1β 119.0318 |
11 | 64.666 | Rb2 | C53H90O22 | 1077.5868 [M−H]− | 1077.5868 [M−H]−; 2,5A1β 101.7272; 0,4A2α 191.0518 |
12 | 65.218 | Rb3 | C53H90O22 | 1077.5876 [M−H]− | 1077.5876 [M−H]−; 945.537 [M−H–Xyl]−; 783.4955 [M−H–Xyl–Glc; 621.4405 [M−H–Xyl–GlcGlc]−; B2α 293.0941 [XylGlc–H]−; C1α 149.0445 [Xyl–H]− |
13 | 68.840 | Rd | C48H82O18 | 991.5492 [M+HCOO]− 945.4906 [M−H]− | 991.5492 [M+HCOO]−; 945.4906 [M−H]−; 783.4501 [M−H–Glc]−; 621.4348 [M−H–GlcGlc]−; B1α/B1β 161.0439 [Glc–H]−; 2,5A1α/2,5A1β 101.0234 |
14 | 70.129 | mRd | C51H84O21 | 1031.5434 [M−H]− | 1031.5434 [M−H]−; 945.5438 [M−H–mal]−; 83.4890 [M−H–mal–Glc]−; 621.4369 [M–mal–H–GlcGlc]−; 459.3829 [M–mal–H–GlcGlcGlc]−; B1α/B1β 161.0433 [Glc–H2O–H]− |
15 | 77.568 | Rg4/Rg6 | C42H70O12 | 811.4891 [M+HCOO]− 765.4832 [M−H]− | 811.4891 [M+HCOO]−; 765.4832 [M−H]−; 457.3645 [M−H–Rha–Glc]−; 1,3A2β 205.0715; 0,2A1β 101.0231 |
16 | 79.547 | Rg4/Rg6 | C42H70O12 | 811.4891 [M+HCOO]− 765.4832 [M−H]− | 811.4891 [M+HCOO]−; 765.4832 [M−H]−; 457.3645 [M−H–Rha–Glc]−; 1,3A2β 205.0715; 0,2A1β 101.0231 |
17 | 87.753 | 20(S)-Rg3 | C42H72O13 | 829.5002 [M+HCOO]− 783.4932 [M−H]− | 829.5002 [M+HCOO]−; 783.4932 [M−H]−; 621.4402 [M−H–Glc]−; 459.3860 [M−H–Glc–Glc]−; 1,3A2β 221.0662; B1β 161.0445 [Glc–H]− |
18 | 88.547 | 20(R)-Rg3 | C42H72O13 | 829.4991 [M+HCOO]− 783.4931 [M−H]− | 829.4991 [M+HCOO]−; 783.4931 [M−H]−; 621.4377 [M−H–Glc]−; 459.3846 [M−H–GlcGlc]−; 1,3A2β 221.0635; B1β 161.0427 [Glc–H2O–H]− |
19 | 94.527 | Rg5/Rk1 | C42H70O12 | 765.4816 [M−H]− | 765.4816 [M−H]−; 603.4279 [M−H–Glc]−; 441.3775 [M−H–GlcGlc]−; 1,3A2β 221.0643; B1β 161.0428 [Glc–H2O–H]− |
20 | 95.286 | Rg5/Rk1 | C42H70O12 | 765.4816 [M−H]− | 765.4816 [M−H]−; 603.4279 [M−H–Glc]−; 441.3775 [M−H–GlcGlc]−; 1,3A2β 221.0643; B1β 161.0428 [Glc–H2O–H]− |