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Fig. 4 | Journal of Translational Medicine

Fig. 4

From: Establishment of an orthotopic patient-derived xenograft mouse model using uveal melanoma hepatic metastasis

Fig. 4

a Two representative examples of histopathological features of patient tumors and the corresponding xenograft tumors. H&E-stained sections and immunostained sections with HMB-45, Melan A, and S-100 antibodies, ×400. Scale bar 50 µm. Patient tumors are depicted in the columns 1 and 4. The corresponding xenograft tumors are depicted in columns 2 and 3, and 5 and 6, respectively. Control panels show no positive signal with an isotype control antibody (inset images located at bottom right in each immunostaining panel). X 0  Patient original tumors, X 1 PDX tumors in the first-generation mice, X 2  PDX tumors in the second-generation mice. b Two representative examples of DNA copy number variation with karyogram. Individual chromosomes are shown in the karyograms, with bars on the right side of the karyograms indicating the chromosomal locations of copy number losses and gains, respectively. Chromosomes 1 to 12 are lined up on the top and chromosome 13 to 22, X and Y are on the bottom. From left to right, patient tumor (X0) is at the left with its adjacent karyogram, the corresponding first-generation xenograft tumor (X1) is in the second column, the corresponding second-generation xenograft (X2) is in the third column, and three different xenograft tumors generated from frozen tumor specimens using F, D and R cryopreservation medium are in the fourth to sixth columns. A copy number of 2 (normal) is indicated by blank space (no color); copy number greater than 2 (chromosomal gain or amplification) is indicated in blue; and copy number less than 2 (chromosomal loss or deletion) is indicated in red

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