Fig. 6
From: Betaine promotes cell differentiation of human osteoblasts in primary culture
Betaine increases SOD2 levels. a Real time PCR. 10 mM BET up regulated significantly SOD2 gene expression after 1 h. b Western blot analysis and relevant quantification: BET increased significantly SOD2 protein content in hObs after 6 and 24 h treatment. c Immunofluorescence analysis (magnification: 40×) and relevant quantification confirmed BET positive action on SOD2 protein level. Data are the mean ± SD of six experiments performed with cells obtained from different donors. ANOVA for non parametric data (Kruskal–Wallis) with Dunn’s multiple comparison test: **p ≤ 0.01 vs control. For western blot-immunofluorescence studies: Student’s t test: *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001