Fig. 3

SHR6390 induced cell cycle arrest at G1 phase in Eca 109 and KYSE-510 cell lines. a Flow cytometric analysis showed that the percentage of cells accumulated in G1 phase significantly increased after treatment with SHR6390 alone compared to the control in Eca 109 and KYSE-510 cell lines not in Eca 9706 cell line. b The expressions of p53 and p21 was further upregulated by SHR6390 concomitant with cell cycle arrest at G1 phase. The data are expressed as the mean ± SD of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 by one-way ANOVA or unpaired two-tailed t test