Fig. 5

Inhibition of necroptosis does not affect shikonin-induced apoptosis. a Cells were transfected with control siRNA or RIP siRNA. After 6 h of transfection, the cells were incubated with 3 or 6 µM shikonin for 3 h before analysis by western blotting. The cell lysates were subjected to 10 or 15% SDS-PAGE to measure the expression of the indicated proteins. Immunoblots are representative of at least two independent experiments. b Necroptosis was evaluated by annexin V/propidium iodide staining. c Values are presented as mean ± SD of three independent experiments. *p < 0.01 compared with the shikonin-treated group